Edward E. Elias S. Arnér, Prof. Péter Nagy, Dr. Éva Dóka, Tamás Ditrói and Dr. Katalin Erdélyi at Semmelweis University. Miklós Geiszt, Semmelweis University, Dept.
Janne Lehtiö, Dept. Ed Schmidt, Dept. John L. Christine Winterbourn, University of Otago Christchurch, Centre for Free Radical Research, Christchurch, New-Zealand Methods and equipment Metabolomics and proteomics platform Enzymatic activity, analysis of various metabolites and reactive sulfur species by mass spectrometry and liquid chromatography Thermo LTQ XL mass spectrometer EASY-nLC II nano HPLC · Thermo Scientific UltiMate HPLC with fluorescent detector Cell and tissue culture Investigation of human cancer cell lines — breast, lung, pancreas, melanoma Development of xenograft models in immune deficient mice Examination of neutrophil granulocytes isolated from human blood plasma Processsing of mouse tissue samples for metabolomics and proteomics sterile cell culture hoods, incubator, hypoxic chamber, microscope, etc.
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Polysulfide generation activity of MPO by customized fluorescent assay Garai et al. PubMed Link.